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DOI: 10.15507/0236-2910.027.201703.428-439
Comparative examination of two methods for modeling autoimmune uveitis
Svetlana V. Aksenova
Professor of Hospital Surgery Chair with Courses of Traumatology, Orthopedics and Ophthalmology, Medical Institute, National Research Mordovia State University (68, Bolshevistskaya St., Saransk 430005, Russia), Dr.Sci. (Medicine), ORCID: http://orcid.org/0000-0002-0186-5044, This email address is being protected from spambots. You need JavaScript enabled to view it.
Nikolay A. Pyatayev
Professor of Anaesthesiology and Resuscitation Chair with courses of Health Science, Health, Safety and Disaster Medicine, Medical Institute, National Research Mordovia State University (68, Bolshevistskaya St., Saransk 430005, Russia), Dr.Sci. (Medicine), ORCID: http://orcid.org/0000-0002-9688-7640, This email address is being protected from spambots. You need JavaScript enabled to view it.
Marina V. Malkina
Doctoral Student of Hospital Surgery Chair with Courses of Traumatology, Orthopedics and Ophthalmology, Medical Institute, National Research Mordovia State University (68, Bolshevistskaya St., Saransk 430005, Russia), ORCID: http://orcid.org/0000-0002-2387-7097, This email address is being protected from spambots. You need JavaScript enabled to view it.
Anastasiya V. Sedoykina
Doctoral Student of Hospital Surgery Chair with Courses of Traumatology, Orthopedics and Ophthalmology, Medical Institute, National Research Mordovia State University (68, Bolshevistskaya St., Saransk 430005, Russia), ORCID: http://orcid.org/ 0000-0001-9969-2075, This email address is being protected from spambots. You need JavaScript enabled to view it.
Nadezhda V. Lakshtankina
Intern of Hospital Surgery Chair with Courses of Traumatology, Orthopedics and Ophthalmology, Medical Institute, National Research Mordovia State University (68, Bolshevistskaya St., Saransk 430005, Russia), ORCID: http://orcid.org/0000-0002-7030-5737, This email address is being protected from spambots. You need JavaScript enabled to view it.
Sergey A. Firstov
Postgraduate of Anaesthesiology and Resuscitation Chair with Courses of Health Science, Health Safety, and Disaster Medicine, Medical Institute, National Research Mordovia State University (68, Bolshevistskaya St., Saransk 430005, Russia),ORCID: http://orcid.org/0000-0003-1961-7708, This email address is being protected from spambots. You need JavaScript enabled to view it.
Oleg A. Kulikov
Associate Professor of the Department of Pharmacology and Clinical Pharmacology with Courses of Pharmaceutical Technology, Medical Institute, National Research Mordovia State University, (68, Bolshevistskaya St., Saransk 430005, Russia), ORCID: http://orcid.org/0000-0003-4411-677X, This email address is being protected from spambots. You need JavaScript enabled to view it.
Yekaterina P. Brodovskaya
Postgraduate Student of Anaesthesiology and Resuscitation Department with Courses of Health Science, Health Safety, and Disaster Medicine, Institute of Medicine, National Research Mordovia State University (68 Bolshevistskaya St., Saransk 430005, Russia), ORCID: http://orcid.org/0000-0002-1060-9843, This email address is being protected from spambots. You need JavaScript enabled to view it.
Introduction: Uveitis is a disease of the uveal tract, characterized by a variety of causes and clinical manifestations. The internal antigens prevail often in the pathogenesis of the disease and develop the so-called autoimmune reactions. The uveitis treatment has an important medico-social significance because of the high prevalence of uveitis, the significant rate of the disease in young people, and high disability. The article compares the efficiency of two methods for modeling autoimmune uveitis.
Materials and Methods: The research was conducted on 6 rabbits of the Chinchilla breed (12 eyes). Two models of experimental uveitis were reproduced on rabbits using normal horse serum during the research. A clinical examination of the inflammatory process course in the eyes was carried out by biomicroscopy using a slit lamp, and a direct ophthalmoscope. Histological and immunological examinations were conducted by the authors of the article.
Results: The faster-reproducing and vivid clinical picture of the disease was observed in the second group. The obvious changes in the immunological status of the animals were noted also: an increase in the number of leukocytes, neutrophils, HCT-active neutrophils, and activation of phagocytosis.
Discussion and Conclusions: The research has showed that the second model of uveitis is the most convenient working variant, which is characterized by high activity and duration of the inflammatory process in the eye.
Keywords: autoimmune uveitis, model of experimental uveitis, immunological status, normal horse serum, resolving dose, intravitreal injection
For citation: Aksenova S. V., Pyatayev N. A., Malkina M. V., Lakshtankina N. V., Sedoykina A. A., Firstov S. A., Kulikov O. A., Borodskaya Ye. P. Comparative examination of two methods for modeling autoimmune uveitis. Vestnik Mordovskogo universiteta = Mordovia University Bulletin. 2017; 27(3):428–439. DOI: 10.15507/0236-2910.027.201703.428-439
Contribution of the co-authors: S. V. Aksenova and N. A. Pyatayev: scientific management, formulating the research problem, development of the theoretical framework, critical analysis and revision of the draft; M. V. Malkina, N. V. Lakshtankin and S. V. Firstov: data collecting and analyzing, finalization of the solution; O. A. Kulikov and Ye. P. Brodovskaya: reviewing and analyzing the scientific literature on research topic, computer data processing.
This work is licensed under a Creative Commons Attribution 4.0 License.
