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DOI: 10.15507/0236-2910.027.201703.440-451
Musculus soleus of rats at physical activity and L-carnitine and creatine phosphate effect
Irina A. Khutorskaya
Assistant of Chair of Cytology, Histology and Embryology with Courses of Medical Biology and Molecular Biology of Cell, National Research Mordovia State University (68 Bolshevistskaya St., Saransk 430005, Russia), ORCID: http://orcid.org/0000-0001-5553-0525, This email address is being protected from spambots. You need JavaScript enabled to view it.
Vladimir P. Balashov
Head of Chair of Cytology, Histology and Embryology with Courses of Medical Biology and Molecular Biology of Cell, National Research Mordovia State University (68 Bolshevistskaya St., Saransk 430005, Russia), Dr.Sci. (Biology), Professor, ORCID: http://orcid.org/0000-0002-9419-1498, This email address is being protected from spambots. You need JavaScript enabled to view it.
Larisa A. Balykova
Director of the Medical Institute, Head of Chair of Pediatrics, National Research Mordovia State University (68 Bolshevistskaya St., Saransk 430005, Russia), Dr.Sci. (Medicine), Professor, Corresponding Member of RAS, ORCID: http://orcid.org/0000-0002-2290-0013, This email address is being protected from spambots. You need JavaScript enabled to view it.
Gulnara F. Shaymardanova
Researcher of Institute of Biochemistry and Biophysics, Kazan Science Centre of the Russian Academy of Sciences (2/31 Lobachevskogo St., Kazan 420111, Russia), Assistant of Chair of Histology, Cytology and Embryology, Kazan State Medical University (49 Butlerova St., Kazan, Russia), Dr.Sci. (Biology), ORCID: http://orcid.org/0000-0003-4472-6674, This email address is being protected from spambots. You need JavaScript enabled to view it.
Alina R. Vasilyeva
Student of Kazan State Medical University (49 Butlerova St., Kazan 420012, Russia), ORCID: http://orcid.org/0000-0001-9779-6823, This email address is being protected from spambots. You need JavaScript enabled to view it.
Svetlana V. Gushchina
Professor of Chair of Cytology, Histology and Embryology with Courses of Medical Biology and Molecular Biology of Cell, National Research Mordovia State University (68 Bolshevistskaya St., Saransk 430005, Russia), Dr.Sci. (Biology), ORCID: http://orcid.org/0000-0002-2314-4774, This email address is being protected from spambots. You need JavaScript enabled to view it.
Tatyana V. Lomonova
Laborotory Assistaint, Histology and Embryology with Courses of Medical Biology and Molecular Biology of Cell, National Research Mordovia State University (68 Bolshevistskaya St., Saransk 430005, Russia), ORCID: http://orcid.org/0000-0001-6601-5043, This email address is being protected from spambots. You need JavaScript enabled to view it.
Introduction: The study of the effect of metabolic drugs on the histochemical characteristics of soleus muscle is relevant for solving the problem of providing the training process in Russia with non-doping drugs for safe correction of the consequences of intense physical activity in athletes.
Materials and Methods: Dynamic physical activity in rats (n = 24) was simulated by swimming “to the limit” with weighting of 10 % of body weight (20 days, 1 time per day). The experimental animals were divided into four groups (6 animals each): № 1 – control, № 2 – swimming + isotonic NaCl solution, № 3 and № 4 – swimming + L-carnitine or creatine phosphate 100.0 mg/kg daily intraperitoneally. The object of the study was musculus soleus. Differentiation of muscle fibers was carried out by the intensity of histochemical activity of succinate dehydrogenase (SDG) and alkaline stable adenosine triphosphate (ATP) of myosin. The percentage of muscle fibers was evaluated and their diameter was defined by the direct morphometry. The obtained data were treated statistically by Student’s T-test.
Results: Swimming of the animals “to the limit” do not affect the ratio of fibers with different phenotypes in the soleus muscle. This indicator is genetically determined and was not modified by L-carnitine and creatine phosphate. Dynamic physical activity promotes the development of hypertrophy of muscle fibers of various types. The investigated medicaments of the metabolic type either do not influence on the formation of exerciseinduced hypertrophy (predominantly creatine phosphate) or reduce the intensity of the hypertrophic process (predominantly L-carnitine) under dynamic physical activity.
Discussion and Conclusions: The obtained data indicate L-carnitine and creatine phosphate do not have an anabolic effect. Taking into account the relevant data on ability of L-carnitine and creatine phosphate to effectively correct a negative effects of intensive physical exercises, these medicaments can be considered as a medications without anabolic properties for various levels athletes.
Keywords: musculus soleus, L-carnitine, creatine phosphate, physical activity, succinate dehydrogenase, adenosine triphosphatease оf myosin
For citation: Khutorskaya I. A., Balashov V. P., Balykova L. A., Shaymardanova G. F., Vasilyeva A. R., Lomonova T. V. Musculus soleus of rats at physical activity and L-carnitine and creatine phosphate effect. Vestnik Mordovskogo universiteta = Mordovia University Bulletin. 2017; 27(3):440–451. DOI: 10.15507/0236-2910.027.201703.440-451
Contribution of the co-authors: I. A. Khutorskaya: conducting the experimental work, writing the draft, analyzing the relevant literature; V. P. Balashov: managing the research project, developing the theoretical framework and methods of research, critical reviewing the final text; L. A. Balykova: developing the theoretical framework, critical the final text; G. F. Shaymardanova: reviewing the relevant literature and data processing; A. R. Vasilyeva: formatting the final text, statistical data processing; S. V. Gushchina: reviewing the relevant literature and analyzing the data; T. V. Lomonova: carried out experimental work, statistical data processing.
This work is licensed under a Creative Commons Attribution 4.0 License.
