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DOI: 10.15507/2658-4123.034.202402.281-294

 

Optical Photoluminescent Properties of Plant Seeds when Infected with Mycopathogens

 

Mikhail V. Belyakov
Dr.Sci. (Eng.), Associate Professor, Chief Researcher of the Laboratory of Innovative Technologies and Technical Means of Feeding in Animal Husbandry, Federal Scientific Agroengineering Center VIM (5, 1st Institutskiy Proyezd, Moscow 109428, Russian Federation), ORCID: https://orcid.org/0000-0002-4371-8042, Researcher ID: ABB-2684-2020, This email address is being protected from spambots. You need JavaScript enabled to view it.

Maksim N. Moskovskiy
Dr.Sci. (Eng.), Professor of the Russian Academy of Sciences, Chief Researcher of the Laboratory of Technologies and Machines for Post-Harvest Processing of Grain and Seeds, Federal Scientific Agroengineering Center VIM (5, 1st Institutskiy Proyezd, Moscow 109428, Russian Federation), ORCID: https://orcid.org/0000-0001-5727-8706, Researcher ID: L-5153-2017, This email address is being protected from spambots. You need JavaScript enabled to view it.

Igor Yu. Efremenkov
Specialist of the Laboratory of Innovative Technologies and Technical Means of Feeding in Animal Husbandry, Federal Scientific Agroengineering Center VIM (5, 1st Institutskiy Proyezd, Moscow 109428, Russian Federation), ORCID: https://orcid.org/0000-0003-2302-9773, Researcher ID: AGR-5540-2022, This email address is being protected from spambots. You need JavaScript enabled to view it.

Vasiliy S. Novikov
Cand.Sci. (Phys.-Math.), Researcher at the Laboratory of Technologies and Machines for Post-harvest Processing of Grain and Seeds, Federal Scientific Agroengineering Center VIM (5, 1st Institutskiy Proyezd, Moscow 109428, Russian Federation), ORCID: https://orcid.org/0000-0002-3304-1568, Researcher ID: H-8443-2018, This email address is being protected from spambots. You need JavaScript enabled to view it.

Sergey M. Kuznetsov
Cand.Sci. (Phys.-Math.), Researcher at the Laboratory of Technologies and Machines for Post-Harvest Processing of Grain and Seeds, Federal Scientific Agroengineering Center VIM (5, 1st Institutskiy Proyezd, Moscow 109428, Russian Federation), ORCID: https://orcid.org/0000-0002-8378-7085, Researcher ID: H-9433-2018, This email address is being protected from spambots. You need JavaScript enabled to view it.

Andrey A. Boyko
Cand.Sci. (Eng.), Associate Professor of the Chair of Technical Operation of Aircraft and Ground Equipment, Don State Technical University (1 Gagarin Square, Rostov-on-Don 344000, Russian Federation), ORCID: https://orcid.org/0000-0003-0890-9617, Researcher ID: ABD-3703-2020, This email address is being protected from spambots. You need JavaScript enabled to view it.

Stanislav M. Mikhailichenko
Cand.Sci. (Eng.), Associate Professor of the Chair of Agricultural Machines, Russian Timiryazev State Agrarian University (49 Timiryazevskaya St., Moscow 127434, Russian Federation), ORCID: https://orcid.org/0000-0002-2305-2909, Researcher ID: IQW-4878-2023, This email address is being protected from spambots. You need JavaScript enabled to view it.

Abstract
Introduction. Using digital technologies such as optical monitoring of grain quality will reduce losses of grain crops caused by infection with mycopathogens.
Aim of the Study. The study is aimed at investigating spectral characteristics, excitation parameters and luminescence of cereal seeds when infected with mycopathogens to determine informative spectral ranges and subsequent development of infection control methods.
Materials and Methods. In the study, there were used wheat and barley seeds inoculated with Fusarium graminearum, Alternaria alternata. Excitation and luminescence registra- tion spectra were measured by a diffraction spectrofluorimeter CM 2203 in the range of 230–600 nm. Integral and statistical parameters of spectra were calculated with the use of Microcal Origin program.
Results. It was found that the spectral absorbency of seeds decreases when infected with mycopathogens. For wheat, the integral absorption parameters decrease more significantly when infected with alternaria, and for barley, on the contrary, a greater decrease occurs when infected with fusarium. In the area of 230–310 nm, new excitation maxima appear in infected seeds. When excited by radiation with a wavelength of λ = 284 nm, the spectral and integral characteristics and parameters of infected seeds exceed those for uninfected ones. When excited with 424 nm and 485 nm radiation, the number of disease-free seeds of both wheat and barley exceeds the number of infected seeds.
Discussion and Conclusion. The changes in excitation and photoluminescence spectra can be explained by the substitution of polysaccharides and proteins during mycoculture uptake and modification. To objectively monitor the mycopathogen infestation of seeds, it is advisable to use a photoluminescence range of 290–310 nm when excited by radiation of about 284 nm. To determine if the infection caused with fusarium or alternariasis, photoluminescence monitoring should be used in the range of 380–410 nm.

Keywords: seeds, mycopathogens, optical spectra, photoluminescence, alternariasis, fusariasis, Fusarium graminearum, Alternaria alternata

Conflict of interest: The authors declare no conflict of interest.

Acknowledgments: The authors are grateful to the reviewers, whose critical evaluation of the presented materials and suggestions for improvement contributed significantly to the quality of this article.

For citation: Belyakov M.V., Moskovskiy M.N., Efremenkov I.Yu., Novikov V.S., Kuznetsov S.M., Boyko A.A., et al. Optical Photoluminescent Properties of Plant Seeds when Infected with Mycopathogens. Engineering Technologies and Systems. 2024;34(2):281‒294. https://doi.org/10.15507/2658-4123.034.202402.281-294

Authors contribution:
M. V. Belyakov – analyzing literary data, describing the methods and technique of preliminary processing, editing the text, drawing conclusions, drawing the conclusions.
M. N. Moskovskiy – scientific guidance, forming the structure of the article, revising the initial text, critical analysis.
I. Yu. Efremenkov – making measurements and calculations, preparing the initial version of the text and illustrations.
V. S. Novikov – making measurements and calculations, finalizing the initial text.
S. M. Kuznetsov – making measurements and calculations, finalizing the initial text.
A. A. Boyko – describing the methods and technique of preliminary processing.
S. M. Mikhailichenko – analyzing the literary data, drawing the conclusions.

All authors have read and approved the final manuscript.

Submitted 16.10.2023; revised 10.01.2024;
accepted 25.01.2024

 

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