PDF To download article.

DOI: 10.15507/2658-4123.032.202202.295-312

 

“Gradient” Experiment in Horticulture Lighting

 

Elena N. Rakutko
Researcher, Institute for Engineering and Environmental Problems in Agricultural Production ‒ Branch of Federal Scientific Agroengineering Center VIM (3 Filtrovskoye Shosse, Saint Petersburg 196625, Russian Federation), ORCID: https://orcid.org/0000-0002-4355-3866, Researcher ID: AAW-6856-2021, This email address is being protected from spambots. You need JavaScript enabled to view it.

Sergey A. Rakutko
Chief Researcher, Head of the Laboratory of Energy Ecology of Light Culture, Institute for Engineering and Environmental Problems in Agricultural Production ‒ Branch of Federal Scientific Agroengineering Center VIM (3 Filtrovskoye Shosse, Saint Petersburg 196625, Russian Federation), Dr.Sci. (Engr.), Associate Professor, ORCID: https://orcid.org/0000-0002-2454-4534, Researcher ID: B-2745-2014, Scopus ID: 26040971100, This email address is being protected from spambots. You need JavaScript enabled to view it.

Abstract 
Introduction. A characteristic feature of most experimental research works on horticulture lighting is the need to vary the main parameters of the light regime while maintaining other environmental factors at the same level throughout the experiment. The approach to creating the requisite variety of variations of light regime parameters in one cycle of plant cultivation, considered in this work, may be acceptable for reducing the time of experiments or for exploratory works. The aim of the work is to show the possibility of using the illuminance gradient as a source of variation in its value in experimental works on horticulture lighting.
Materials and Methods. We used an LED lamp with a cosine light spatial distribution, placed above the work surface, on which containers with sweet pepper plants were placed. The optical density of the leaves in various spectral ranges was used as a biometric parameter characterizing the response of a plant to the level of illumination.
Results. A significant gradient of illumination was observed on the working surface. With the same range of illumination, the number of containers with plants for a given zone is different and is sufficient to test statistical hypotheses. The values of the average illumination by zones provided the range of illumination change by 2.5 times. The standard deviations of illumination by zones were 97–163 lux and were practically no correlated with the values of the average illumination.
Discussion and Conclusion. The developed mathematical model of a gradient experiment in horticulture lighting makes it possible to determine the possible number of replicates during the experiment, the average illumination value, the standard deviation, the coefficients of variation and uniformity in each zone of plant placement, based on the lighting and layout parameters of the lighting installation. It has been experimentally found that the use of a luminaire with a cosine light distribution provides an illumination gradient on a horizontal surface, the average values of which in individual zones form a linear illumination scale. Coefficients of illumination variation in individual lighting zones with the parameters set in the example were 3.0–11.5%. The coefficients of variation of the optical density of the leaves of pepper plants grown under the conditions of the gradient experiment on illumination were 6.0–11.6%. The differences in the mean values of the optical density of plant leaves in different zones of gradient illumination are statistically significant. This allows us to recommend the use of the proposed method for searching experiments on horticulture lighting.

Keywords: greenhouse horticulture, horticulture lighting, lighting installation, illumination uniformity, gradient, biometry, phenotyping

Conflict of interest: The authors declare no conflict of interest.

For citation: Rakutko E.N., Rakutko S.A. “Gradient” Experiment in Horticulture Lighting. Engineering Technologies and Systems. 2022;32(2):295–312. doi: https://doi. org/10.15507/2658-4123.032.202202.295-312

Contribution of the authors:
E. N. Rakutko – analysis of scientific sources, mathematical modeling, writing the article.
S. A. Rakutko – scientific guidance, formulation of the basic research concept and structure of the article, writing the article.

All authors have read and approved the final manuscript.

Submitted 14.03.2022; approved after reviewing 20.04.2022;
accepted for publication 06.05.2022

 

REFERENCES

1. Janick J. The Origins of Horticultural Technology and Science. In: ISHS Acta Horticulturae 759: XXVII International Horticultural Congress – IHC2006: Global Horticulture: Diversity and Harmony, an Introduction to IHC2006. 2007. Vol. 759. p. 41–60. doi: https://doi.org/10.17660/ActaHortic.2007.759.3

2. Paik I., Huq E. Plant Photoreceptors: Multi-Functional Sensory Proteins and Their Signaling Networks. Seminars in Cell & Developmental Biology. 2019;92:114–121. doi: https://doi. org/10.1016/j.semcdb.2019.03.007

3. Yang D., Seaton D.D., Krahmer J., Halliday K.J. Photoreceptor Effects on Plant Biomass, Resource Allocation, and Metabolic State. Proceedings of the National Academy of Sciences. 2016;113(27):7667–7672. doi: https://doi.org/10.1073/pnas.1601309113

4. Gómez C., Currey C.J., Dickson R.W., et al. Controlled Environment Food Production for Urban Agriculture. Journal of the American Society for Horticultural Science. 2019;54(9):1448–1458. doi: https://doi.org/10.21273/HORTSCI14073-19

5. Cheng G.-X., Zhang R.-X., Liu Sh., et al. Leaf-Color Mutation Induced by Ethyl Methane Sulfonate and Genetic and Physio-Biochemical Characterization of Leaf-Color Mutants in Pepper (Capsicum Annuum L.). Scientia Horticulturae. 2019;257. doi: https://doi.org/10.1016/j.scienta.2019.108709

6. Rajapakse N.C., Li S. Exclusion of Far Red Light by Photoselective Greenhouse Films Reduces Height of Vegetable Seedlings. In: ISHS Acta Horticulturae 631: XXVI International Horticultural Congress: Issues and Advances in Transplant Production and Stand Establishment Research. 2004. Vol. 631. p. 193–199. doi: https://doi.org/10.17660/ActaHortic.2004.631.25

7. López-Marin J., Gálvez A., González A., et al. Effect of Shade on Yield, Quality and Photosynthesis-Related Parameters of Sweet Pepper Plants. In: ISHS Acta Horticulturae 956: VII International Symposium on Light in Horticultural Systems. 2012. Vol. 956. p. 545–552. doi: https://doi.org/10.17660/ActaHortic.2012.956.65

8. Diaz-Perez J.C. Bell Pepper (Capsicum annum L.) Crop as Affected by Shade Level: Microenvironment, Plant Growth, Leaf Gas Exchange, and Leaf Mineral Nutrient Concentration. Journal of the American Society for Horticultural Science. 2013;48(2):175–182. doi: https://doi.org/10.21273/HORTSCI.48.2.175

9. Verkhoturov O.P., Sysoev Ye.V., Khakhalin A.A. [Gradient Compensating Filter]. Patent Application no. 2,002,125,847 Russian Federation. 2004 March 27. 1 p. https://www1.fips.ru/registers-doc-view/fips_servlet?DB=RUPAT&DocNumber=2002125847&TypeFile=html (accessed 29.12.2021). (In Russ.)

10. Kazakov V.I., Rovenskaya T.S., Tochkina G.A. [Gradient Single Lens Micro Lens]. Patent 1,758,622 USSR. 1992 August 30. 6 p. Available at: https://www1.fips.ru/registers-doc-view/fips_servlet?DB=RUPAT&DocNumber=1758622&TypeFile=html (accessed 10.07.2021). (In Russ.)

11. Piepho H.P., Buchse A., Emrich K. A Hitchhiker’s Guide to Mixed Models for Randomized Experiments. Journal of Agronomy and Crop Science. 2003;189(5):310–322. doi: https://doi.org/10.1046/j.1439-037X.2003.00049.x

12. Fernandez G.C.J. Design and Analysis of Commonly Used Comparative Horticultural Experiments. Journal of the American Society for Horticultural Science. 2007;42(5):1052–1069. doi: https://doi.org/10.21273/HORTSCI.42.5.1052

13. Chi Y., Wang E., Wang J. Identifying the Anthropogenic Influence on the Spatial Distribution of Plant Diversity in an Estuarine Island through Multiple Gradients. Global Ecology and Conservation. 2020;21. doi: https://doi.org/10.1016/j.gecco.2019.e00833

14. Garnier E., Navas M.-L. A Trait-Based Approach to Comparative Functional Plant Ecology: Concepts, Methods and Applications for Agroecology. A Review. Agronomy for Sustainable Development. 2012;32:365–399. doi: https://doi.org/10.1007/s13593-011-0036-y

15. Skliar V.G. Using Gradient Analysis in the Study Natural Forest Regrowth. Vіsnik Zaporіzkogo natsіonalnogo unіversitetu. Bіologіchnі nauki. 2015;(2):196–207. Available at: https://www.elibrary.ru/item.asp?id=25038598 (accessed 10.02.2022). (In Ukr., abstract in Eng.)

16. Brüllhardt M., Rotach P., Bigler C., et al. Growth and Resource Allocation of Juvenile European Beech and Sycamore Maple Along Light Availability Gradients in Uneven-Aged Forests. Forest Ecology and Management. 2020;474. doi: https://doi.org/10.1016/j.foreco.2020.118314

17. Leeflang L., During H.J., Werger M.J.A. The Role of Petioles in Light Acquisition by Hydrocotyle Vulgaris L. in a Vertical Light Gradient. Oecologia. 1998;117:235–238. doi: https://doi.org/10.1007/s004420050653

18. Wang S., Zhou D.-W. Architectural Plasticity in Response to Population Density in Abutilon Theophrasti (Malvaceae). Ecological Research. 2022;37(2):228–239. doi: https://doi.org/10.1111/1440-1703.12284

19. Gaynutdinov I.A., Abdullin Sh.R. Gradient Analysis of the Light Influence on the Composition of Cyanobacteria and Algae Coenoses at the Entrance Shaft of the Kutuk-Sumgan Cave (Republic of Bashkortostan). Herald of the Academy of Sciences of the Republic of Bashkortostan. 2016;21(2):11–15. Available at: https://www.elibrary.ru/item.asp?id=26082250 (accessed 10.02.2022). (In Russ., abstract in Eng.)

20. Xu Y. Seven Dimensions of Light in Regulating Plant Growth. In: ISHS Acta Horticulturae 1134: VIII International Symposium on Light in Horticulture. 2016. Vol. 1134. p. 445–452. doi: https://doi.org/10.17660/ActaHortic.2016.1134.56

21. Semenova N.A., Grishin A.A., Dorokhov A.A. Analytical Review of Climatic Chambers for Vegetable Crops Growing. Bulletin NGII. 2020;(1):5–15. Available at: https://www.elibrary.ru/item.asp?id=41863682 (accessed 10.02.2022). (In Russ., abstract in Eng.)

22. Petrenko E.E., Stepanchuk G.V., Klyuchka Ye.P., Ponomareva N.Ye. [Multi-Level Shelving for Research Work]. Patent 2,537,923 Russian Federation. 2015 Junuary 10. 5 p. Available at: https://www1.fips.ru/registers-doc-view/fips_servlet?DB=RUPAT&DocNumber=2537923&TypeFile=html (accessed 20.12.2021). (In Russ.)

23. Martirosyan Yu.T., Martirosyan L.Yu., Martirosyan D.Yu., Akopyan V.B. [Gradient Phytotron]. Patent 207,773 Russian Federation. 2021 November 16. 2 p. Available at: https://www1.fips.ru/registers-doc-view/fips_servlet?DB=RUPM&DocNumber=207773&TypeFile=html (accessed 20.12.2021). (In Russ.)

24. Park Y.H., Park J.K. Light Gradient-Based Screening of Arabidopsis Thaliana on a 384-Well Type Plant Array Chip. Micromachines. 2020;11(2). doi: https://doi.org/10.3390/mi11020191

25. Lejeune P., Fratamico A., Bouché F., et al. LED Light Gradient as a Screening Tool for Light Quality Responses in Model Plant Species. BioRxiv. 2020. doi: https://doi.org/10.1101/2020.10.08.320002

26. Rakutko Ye.N., Rakutko S.A., Vaskin A.V. Effect of Lighting Fixtures Spatial Arrangement on Energy and Ecological Performance of Greenhouse Horticulture. Agroekoinzheneriya. 2021;(3):33–51. (In Russ., abstract in Eng.) doi: https://doi.org/10.24412/2713-2641-2021-3108-33-50

27. Li L., Tian S.L, Jiang J., Wang Y. Regulation of Nitric Oxide to Capsicum under Lower Light Intensities. South African Journal of Botany. 2020;132:268–276. doi: https://doi.org/10.1016/j.sajb.2020.05.020

28. Jacquemoud S., Ustin S. Leaf Optical Properties. Cambridge: Cambridge University Press; 2019. 556 p. doi: https://doi.org/10.1017/9781108686457

29. Rakutko Ye.N., Rakutko S.A., Mishanov A.P., Markova A.Ye. Digital Twin of a Plant in Greenhouse Horticulture: Case Study of Pepper (Capsicum Annuum L.) in Transplant Period. Agroekoinzheneriya. 2021;(3):13–33. (In Russ., abstract in Eng.) doi: https://doi.org/10.24412/2713-2641-2021-3108-13-33

30. Rakutko S.A., Rakutko E.N. Assessment of Lighting Uniformity as a Factor of Energy Efficiency in Greenhouse Horticulture. Engineering Technologies and Systems. 2021;31(3):470–486. (In Russ., abstract in Eng.) doi: https://doi.org/10.15507/2658-4123.031.202103.470-486

 

Лицензия Creative Commons
This work is licensed under a Creative Commons Attribution 4.0 License.

Joomla templates by a4joomla