08. Mamayevа SN, Munkhalova YaA, Kononova IV, Dyakonov AA, Koryakina VN, Shutova VV, Maksimov GV. The study of red blood cells by scanning electron microscopy.

UDC: 616.15-07:615.84

DOI: 10.15507/0236-2910.026.201603.381-390

THE STUDY OF RED BLOOD CELLS BY SCANNING ELECTRON MICROSCOPY

Sargylana N. Mamayeva
docent of General and Experimental Physics chair, Physical Technical Institute, Ammosov North-Eastern Federal University (58, Belinskogo St., Yakutsk, Russia), Ph.D. (Physics and Mathematics), This email address is being protected from spambots. You need JavaScript enabled to view it.

Yana A. Munkhalova
head of Pediatrics and Pediatric Surgery chair, Medical Institute, Ammosov North-Eastern Federal University (58, Belinskogo St., Yakutsk, Russia), Ph.D. (Medicine), This email address is being protected from spambots. You need JavaScript enabled to view it.

Irina V. Kononova
head of Teaching and Research of Clinical Immunology Laboratory, Medical Institute, Ammosov North-Eastern Federal University (58, Belinskogo St., Yakutsk, Russia), This email address is being protected from spambots. You need JavaScript enabled to view it.

Afanasiy A. Dyakonov
leading engineer of Macromolecular Compounds and Organic Chemistry chair, Institute of Natural Sciences, Ammosov North-Eastern Federal University (58, Belinskogo St., Yakutsk, Russia), This email address is being protected from spambots. You need JavaScript enabled to view it.

Veronika N. Koryakina
student of Physical Technical Institute, Ammosov North-Eastern Federal University (58, Belinskogo St., Yakutsk, Russia), This email address is being protected from spambots. You need JavaScript enabled to view it.

Vitalina V. Shutova
docent of Biotechnology, Bioengineering and Biochemistry chair, National Research Mordovia State University (68, Bolshevistskaya St, Saransk, Russia), Ph.D. (Biology), ORCID: http://orcid.org/0000-0001-6437-3621, This email address is being protected from spambots. You need JavaScript enabled to view it.

Georgiy V. Maksimov
professor of Biophysics chair, Lomonosov Moscow State University (1/12, Leninskiye gory, Moscow, Russia), Dr.Sci. (Biology), ORCID: http://orcid.org/0000-0002-7377-0773, This email address is being protected from spambots. You need JavaScript enabled to view it.

Introduction: Using the possibilities of modern equipment, such as a scanning electron microscope (SEM), could provide important additional information for red blood cells (RBCs) diagnostics, which can be used to introduce new methods of complex studies of blood tests in the diagnosis of renal disease with hematuria syndrome.
Materials and Methods: Investigations of the morphology the RBCs of patients with hematuria by SEM high-resolution instrument JSM-7800F. The technique of cell and uncoated nanoparticles research without conductive coatings has been developed.
Results: The findings obtained results of a comparative analysis of the methods of mathematical statistics, linear dimensions of the red blood cells of children Berger’s disease patients and control group. Nanoscale particles detected on the surface of red blood cells and IgA-nephropathy. Smear of blood samples showed that in certain diseases of the observed changes in the morphology of red blood cells and the presence on their surface nano-objects. It can be assumed that these objects are of organic origin, as many organic objects are dielectrics and in the study of SEM, they can be seen as bright glowing objects. Nano-objects have dimensions similar to the small size of viruses and can be a confirmation of the assumption of a possible viral etiological factor Berger disease and other forms of nephropathy. The changes in linear dimensions of erythrocytes upward Berger in diseases compared with the size of the control group and the group of erythrocytes of patients with other forms of nephropathy.
Discussion and Conclusions: The SEM method without spraying of the samples is very accurate for the study of the morphology of blood cells. Its use will improve the quality of diagnosis is difficult diagnosed Berger disease. A further increase in statistics and the analysis of nanometer-sized objects extend the knowledge of the manifestations of the disease.

Keywords: erythrocytes, red blood cells (RBCs), hematuria, Berger’s disease, electron microscopy, nephropathy

For citation: Mamayevа SN, Munkhalova YaA, Kononova IV, Dyakonov AA, Koryakina VN, Shutova VV, Maksimov GV. The study of red blood cells by scanning electron microscopy. Vestnik Mordovskogo universiteta = Mordovia University Bulletin. 2016; 3(26):381-390. DOI: 10.15507/0236-2910.026.201603.381-390

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